In a line structured light measurement system, the accurate extraction of the light stripe centers is one of the key factors affecting the accuracy of the entire system. For the problem of light stripe center extraction in optical measurements in complex environment with high reflection, a multi-scale analysis based method that realizes high-precision extraction of light stripe centers is proposed. The skeleton algorithm is applied to determine the initial centers of the laser stripe, and the mean square deviation σ of the Gaussian kernel is determined based on the width of the light stripe at each initial center. Multi-scale image convolution is carried out to determine the optimal σ, in order to further improve the accuracy of extracting light stripe center. Interlinkage of the light stripe centers is finished. The results show that the method is of high noise immunity, and can achieve high-precision results when the width of the light stripe changes sharply.