Peri-implantitis can lead to peri-implant attachment loss, bone absorption, and even implant loose or lost. In this study, the atomic layer deposition technique and silanization were applied to prepare a composite zinc oxide (ZnO) /fibronectin (Fn) coating on the surface of titanium (Ti). Peri-implantitis related bacteria, including Aggregatibacter actinomycetemcomitans (A. a) and Porphyromonas gingivalis (P. g), were chosen to evaluate the antibacterial effect of the composite coating. Spreading, proliferation and associated gene expression of human gingival fibroblasts on Ti, Ti/ZnO, and Ti/ZnO/Fn were evaluated. In vivo peri-implantitis model was established on SD rat maxilla through the infusion of mixed bacterial suspension. The peri-implant bone resorption and inflammatory infiltration in gingiva were investigated. The results showed that antibacterial efficacies of the composite coating against A. a and P. g for 24 h were up to 80.9% and 75.7%, respectively. Fibroblasts on the surface of Ti/ZnO/Fn showed adhesion and proliferation in vitro. In the model of peri-implantitis, Micro-CT showed that the amount of peri-implant bone absorption of Ti was (1.14±0.71) mm, while the amount of Ti/ZnO/Fn was only (0.37±0.28) mm. Moreover, the observation of paraffin section and qPCR revealed that inflammation level of gingiva and alveolar bone around Ti was lower in Ti/ZnO/Fn than in other groups. In conclusion, the composite coating for dental implants might be effective on defensing bacterial invasion, enhancing gingiva attachment and preventing inflammation response of gingiva.