In this study, the calibration tests were conducted on the Hyperion hyperspectral remote sensor using the automated observation equipment at the Dunhuang radiometric calibration site. This paper details the principle and method of automated calibration and specifies the applied calibration process. A reference reflectance database was established to solve the problem of mismatch between the channel reflectance in the hyperspectral remote sensor and the spectral shape of the reference reflectance. The results of the four effective automated calibration tests performed on Hyperion from October 2016 to April 2017 were compared with the apparent radiance observed by Hyperion. The results show that the relative deviation between the apparent radiance obtained from the site-automated calibration and that from the satellite observation is less than 5% with a standard deviation of less than 2.3% within the 420-1044 nm spectral range. The site-automated calibration results are found to be highly consistent and stable with respect to the hyperspectral satellite observations, indicating that the proposed method can be applied to the high-frequency on-orbit radiometric calibration of hyperspectral remote sensors.