An optical system for the non-mydriatic stereoscopic imaging fundus camera with a field of view of 30° is designed, which is composed of an imaging system and an illumination system. In the imaging system, a new optical structure for the stereoscopic imaging of fundus is designed, in which a prefixing objective lens is introduced to improve the imaging resolution. In the illumination system, an annular aperture is introduced to avoid the generation of the corneal reflected light and the black spot plates are adopted to eliminate the stray light produced by the ophthalmic lens. The research results show that, this system can be used to achieve not only the multi-angle synchronous acquisition of the retina images, but also the highly clear imaging of the fundus with 6×10 6 pixel. As for this system, the object resolution of the normal human eyes is higher than 200 lp·mm -1. This system has an overall length of 290 mm, a field curve value of smaller than 28 μm, and a distortion value of only -4.9%. This system has a strong focusing ability and the human fundus with a diopter range of -7-+5 m -1 can be imaged clearly.