For non-scanning laser three-dimensional (3D) imaging sensors, the imaging resolution is low and the calibration accuracy is not high. In order to solve this problem, a low-resolution sensor calibration method based on bilinear interpolation is proposed．Firstly, a resampling strategy based on the bilinear interpolation algorithm is used to improve the image resolution. Then calibration algorithm based on OpenCV is adopted. Finally the calibration results are compared with those obtained by the traditional calibration method. Experimental results show that the calibration error is reduced by nearly 1/2. The proposed calibration method based on bilinear interpolation algorithm can improve the calibration accuracy of low-resolution sensor.