Mingjie Zheng, Zhifang Li. Spatial-Light Interference Microscope Technology Using Green-Light[J]. Laser & Optoelectronics Progress, 2020, 57(13): 131801
- Laser & Optoelectronics Progress
- Vol. 57, Issue 13, 131801 (2020)
Fig. 1. Sketch of the optical path of Zernike phase-contrast microscope
Fig. 2. Optical path of the proposed green light SLM. (a) Schematic of light path; (b) real optical path
Fig. 3. Spectrum of green light and the corresponding coherent degree curve. (a) Spectrum of green light; (b) coherent degree curve
Fig. 4. Phase distribution of a standard 6 μm oil immersed PS microsphere
Fig. 5. Bright field microscopic images of PS microsphere. (a) Water immersed microsphere; (b) oil immersed microsphere
Fig. 6. Grating used for the SLM alignment, the objective back pupil image after alignment, and the ring light source used in the phase contrast imaging. (a) Grating; (b) back pupil image; (c) ring light source
Fig. 7. Four ring gratings used in the SLIM imaging and the corresponding SLIM images of microspheres. (a) Gratings; (b) corresponding microsphere SLIM images
Fig. 8. Four section curves corresponding to the four microspheres showed in Fig. 7 . (a) I1 with phase modulation angle of 0(0°); (b) I2 with phase modulation angle of π/2(90°); (c) I3 with phase modulation angle of π(180°); (d) I4 with phase modulation angle of 3π/2(270°)
Fig. 9. Relationship between correction function and phase difference from -10π to 10π
Fig. 10. Reconstructed images of the oil immersed microsphere. (a) 2D contour of the wrapped phase of the oil immersed microsphere; (b) 3D contour of the unwrapped phase of the oil immersed microsphere
Fig. 11. Comparing the x direction OPD distribution of the oil immersed microsphere (solid line) with that of the standard situation (dashed line). (a) Processed with applying median filter; (b) processed with applying median filter and coherent degree correction
Fig. 12. Red cell image captured in the SLIM experiment
Fig. 13. Red cell image after phase reconstruction. (a) 2D contour of wrapped phase of the red cell; (b) 3D contour of unwrapped phase distribution of the red cell
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Table 1. Comparison of the calculation results using different processing methods
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