• Acta Optica Sinica
  • Vol. 39, Issue 2, 0217001 (2019)
Han Sun1、2、* and Tongsheng Chen2、*
Author Affiliations
  • 1 School of Information and Optoelectronic Science and Engineering, South China Normal University, Guangzhou, Guangdong 510006, China
  • 2 College of Biophotonics, South China Normal University, Guangzhou, Guangdong 510631, China
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    DOI: 10.3788/AOS201939.0217001 Cite this Article Set citation alerts
    Han Sun, Tongsheng Chen. Automatic Background Recognition and Data Selection for Online Quantitative E-FRET Imaging[J]. Acta Optica Sinica, 2019, 39(2): 0217001 Copy Citation Text show less
    Statistical pixel grayscale values of images and corrected images
    Fig. 1. Statistical pixel grayscale values of images and corrected images
    Quantitative E and RC. (a) Images; (b) corresponding histograms
    Fig. 2. Quantitative E and RC. (a) Images; (b) corresponding histograms
    E and RC results after data filtering with different Boolean logic templates. (a) Bmask; (b) E; (c) RC
    Fig. 3. E and RC results after data filtering with different Boolean logic templates. (a) Bmask; (b) E; (c) RC
    E and RC images obtained by online dynamic detection
    Fig. 4. E and RC images obtained by online dynamic detection
    E and RC values according to Fig. 4
    Fig. 5. E and RC values according to Fig. 4
    βERC
    MeanSDMeanSD
    00.28350.11461.121.15
    10.31870.03631.130.07
    20.30190.02931.140.05
    30.31230.02661.140.04
    Notes: reported E[16] is 0.299±0.004, and expected RC is 1.
    Table 1. E and RC after template filtering with different β
    PlasmidβERC
    Mean valueSDReported valueMean valueSDExpected value
    C4Y00.32000.11440.299±0.0041.280.961
    10.31650.03961.140.08
    20.29110.02771.130.07
    C10Y00.23910.07360.223±0.0031.270.701
    10.23010.03461.150.11
    20.22060.03821.090.09
    C40Y00.14300.08510.158±0.0021.130.911
    10.14440.03621.100.13
    20.14860.03191.090.10
    C80Y00.09070.05340.116±0.0021.230.921
    10.08400.02151.100.13
    20.08520.01961.080.07
    Table 2. E-FRET quantitative imaging results of different plasmids
    Han Sun, Tongsheng Chen. Automatic Background Recognition and Data Selection for Online Quantitative E-FRET Imaging[J]. Acta Optica Sinica, 2019, 39(2): 0217001
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