• Spectroscopy and Spectral Analysis
  • Vol. 40, Issue 11, 3477 (2020)
Meng-yao LI1、1、*, Shu-ya WANG1、1, Yun-feng XIE1、1, Yun-guo LIU1、1, and Chen ZHAI1、1
Author Affiliations
  • 1[in Chinese]
  • 11. Nutrition & Health Research Institute, COFCO Corporation, Beijing Key Laboratory of Nutrition & Health and Food Safety, Beijing 102209, China
  • show less
    DOI: 10.3964/j.issn.1000-0593(2020)11-3477-06 Cite this Article
    Meng-yao LI, Shu-ya WANG, Yun-feng XIE, Yun-guo LIU, Chen ZHAI. Detection of Protease Deterioration Factor in Tomato by Fluorescence Sensor Array[J]. Spectroscopy and Spectral Analysis, 2020, 40(11): 3477 Copy Citation Text show less
    (a) Principle of fluorescence immunoassay; (b) Synthesis of probe and its reaction with carboxylesterase; (c) Schematic of simultaneous detection of multiple enzymes
    Fig. 1. (a) Principle of fluorescence immunoassay; (b) Synthesis of probe and its reaction with carboxylesterase; (c) Schematic of simultaneous detection of multiple enzymes
    (a) Fluorescence spectra and linear fitting curve of pectinases at different concentrations; (b) Fluorescence spectra of probe (10 μmol·L-1) reacting with carboxylesterase at different concentrations
    Fig. 2. (a) Fluorescence spectra and linear fitting curve of pectinases at different concentrations; (b) Fluorescence spectra of probe (10 μmol·L-1) reacting with carboxylesterase at different concentrations
    Fluorescence responses of immune-fluorescent probes with other interferences
    Fig. 3. Fluorescence responses of immune-fluorescent probes with other interferences
    酶种类加标量/
    (U·mL-1)
    检测平均值/
    (U·mL-1)
    标准
    偏差
    回收率
    /%
    变异系
    数/%
    PE0.1
    100.0
    300.0
    0.09
    92.1
    277.6
    0.006
    10.4
    23.1
    90.0
    92.1
    92.5
    6.7
    11.3
    8.3
    CAT0.2
    400.0
    600.0
    0.18
    376.0
    568.0
    0.008
    22.9
    45.7
    90.0
    94.0
    94.7
    4.4
    6.1
    8.1
    SOD1.0
    500.0
    600.0
    0.95
    483.0
    572.0
    0.1
    41.5
    47.9
    95.0
    96.6
    95.3
    10.5
    8.6
    8.4
    PPO20.0
    30.0
    50.0
    20.2
    30.6
    49.8
    2.7
    3.1
    3.9
    101.1
    102.3
    99.7
    13.4
    10.1
    7.8
    CXE0.15
    0.2
    0.3
    0.14
    0.18
    0.28
    0.012
    0.017
    0.023
    93.3
    98.0
    93.0
    8.6
    9.1
    8.0
    Table 1. Addition and recycling experiment of sample (n=5)
    Meng-yao LI, Shu-ya WANG, Yun-feng XIE, Yun-guo LIU, Chen ZHAI. Detection of Protease Deterioration Factor in Tomato by Fluorescence Sensor Array[J]. Spectroscopy and Spectral Analysis, 2020, 40(11): 3477
    Download Citation