For the high-speed multi-frame camer used to capture time-resolved sequence images, the main image processing includes geometric distortion calibration,image pixel position arrangement, flat-field correction for photo-response non-uniformity between the pixels in one image and the response calibration between different sequence images．These processings can meet the demand not only on each image itself but also on the morphological comparison of sequence images. The image processing method developed from related methods to solve the above problems provides an effective way to calibrate the non-uniformity and correlation coefficients between images, and scan be used to acquire the uniformity of the sequence images in the multi-images system in the practical applications.