It is intended to obtain the cell contents (protein, DNA, RNA etc.) and probe into the application of optoelectronic technology for crushing cell. In theory BHK21 initial transmembrane voltage is introduced to modify the voltage model. Control and detecting systems for crushing cells are made as follows: crushing cells chip electrodes 50 μm in both width and pitch interval, with a length of 0.4 cm and thickness of 0.5 μm each. The system could be used in cells crushing, controlling and automatic testing. It takes inverted microscope as the main light path system, collects pictures with 3 mega pixel CCD camera and deals them with processing software of micro-digital image. The experiment shows that this optical testing system could automatically identify and count BHK21 in micro-windows. For crushing BHK21 cells, the most suitable voltage range and pulse width are 3 V and 3 ms.