According to spectral characteristics that benzoylurea pesticides can emit fluorescence as they are irradiated by UV rays,an optical fiber fluorescence measurement system based on Charge-coupled Device (CCD) was designed for pesticides residue. The system adopted a pulsed xenon lamp as excitation light-source,employed optical fibers to transmit and detect fluorescence,and used linear-array CCD instead of traditional photomultiplier tube as photoelectric detection device of the fluorescence signal. Moreover,it was equipped with high-speed A/D data acquisition card,implemented photoelectric conversion of the fluorescence and data acquisition under the control of a singlechip,and further achieved the concentration measurement of flufenoxuron and hexaflumuron. The result indicates that,when the excitation wavelength is 290 nm for flufenoxuron and 345 nm for hexaflumuron respectively,the fluorescence intensity of the former appears maximum as the wavelength is 360 nm and that of the latter appears maximum as the wavelength is 418 nm. The minimum detecting limits are 12 μg/L and 20 μg/L respectively. The fluorescence intensity is directly proportional to solution concentration in the range of 25~1 000 μg/L. This measurement system has several characteristics such as high sensitivity and wide linear range,which can meet the demand of fluorescence detection.