Man Peng, Zhongchen Bai, Ying Zhang, Xianji Li, Zhengping Zhang. Protein Detection Chip Based on Quantum Dot Fluorescence Quenching Method[J]. Laser & Optoelectronics Progress, 2019, 56(6): 062601
- Laser & Optoelectronics Progress
- Vol. 56, Issue 6, 062601 (2019)
Fig. 1. Principle of protein-chip detection. (a) Chip structure; (b) detection principle
Fig. 2. Experimental setup. (a) Block diagram; (b) prototype photo
Fig. 3. AFM of glass substrate. (a) Unmodified; (b) modified
Fig. 4. Infrared Fourier transform spectrum of glass substrate modified by APTES
Fig. 5. Nanometer quantum dot biochip array. (a) Surface, the inset is an array unit photo; (b) fluorescence, the inset is a fluorescent image
Fig. 6. Nanometer quantum dot biochip array unit. (a)-(c) Photos; (d)-(f) fluorescent images after binding antibodies; (g)-(i) fluorescent images after binding antigens on antibody substrate
Fig. 7. Relationship between NGF concentration and fluorescence peak intensity. (a) 0-100 ng·mL-1; (b) partial enlargement
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Table 1. Fluorescence quenchingdegrees under different antigen concentrations
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