In order to study the influence of He-Ne laser on "root-bending" in wheat caused by enhanced UV-B irradiation, the wheat seedlings were exposed to He-Ne laser with 5 mW·mm-2 power density after enhanced UV-B irradiation (10.08 kJ·m-2·d-1). The content of actin, the content and type variety of flavonoids in wheat roots were studied. The results show that compared with those of the contrast group, the main radical length becomes shorter, the content of actin and flavonoids in the UV-B irradiation enhanced group is reduced, while the He-Ne laser treated group has no notable difference. The results of the group under simultaneous irradiation of UV-B and He-Ne laser are lower than those of the contrast group but higher than those of the UV-B irradiated group, and the "root bending" phenomenon is weakened. The migration rates of flavonoids are different in diverse groups. There appear various flavonoids varieties under UV-B irradiation. The variation in content of actin and flavonoids is one of the reason for "root bending". The damage on wheat seedlings induced by UV-B irradiation can be partially repaired by He-Ne laser irradiation.