In order to meets the high frame rate and high sensitivity needs of wavefront sensor in adaptive optics systems, an external driving circuit for E2V’s high frame rate and low-light EMCCD chip CCD60 was designed. This system used FPGA to generate the primary control logics and all of timing signals, and used pin driver EL7156 to generate the special driving signals of CCD60 from FPGA’s TTL logical signals. High voltage sine driving signal was used to drive high-voltage gain gate, which was generated by Direct Digital Synthesizer (DDS) and amplified by a novel high-voltage amplifier module. The range of designed high voltage sine driving signal can be real-timely adjusted and worked very well when generating electron gain. The design of the driving circuit has been successfully applied in CCD60 high frame rate and low-light imaging system.