In indoor video viewing scenarios using visual display terminal (VDT), the VDT off-screen background light can reduce the discomfort caused by the contrast between light and dark inside and outside the screen, and the color as well as luminance properties of light will affect visual perception of people. The effect of indoor light environment on the visual perception of dynamic video viewing with VDT was explored, so as to create a VDT viewing light environment that can improve visual perception. The human factor engineering experiments were carried out based on psychophysical method, and the indoor light environment took the color/color temperature and luminance of the light as independent variables. In experiment 1, 3 kinds of color colors and 3 kinds of white color temperatures were set. In experiment 2, 8 kinds of luminance conditions were set, the video materials were categorized as high-dynamic video and low-dynamic video according to dynamic level, and the visual perception of the subjects watching the video using VDT under different light environment conditions was measured. The results show that the colored light is preferred, which conducts lower fatigue, higher comfort and other visual feelings than white light, among which the green light is the most comfortable condition. The luminance range of 9 cd/m²~30 cd/m² is the most preferred, the condition range with best visual perception is 16 cd/m²~54 cd/m², conducting lower fatigue, higher comfort, atmosphere and display color quality, and visual perception is worst when the luminance exceeds 330 cd/m². The experimental results provide a reference for the study of the effect of indoor light environment on human visual perception, and provide practical guidance and suggestions for lighting design for improving visual perception of dynamic video viewing with VDT.