In remote sensing, the subdivision and increase of detecting bands require to enhance the acquisition and transmission speed of image. This makes the old wavelength tuning method and interface specification can not meet the need. A new multispectral image acquisition system based on liquid crystal tunable filter (LCTF) is proposed. In this system, LCTF is used to substitute traditional filter wheel. It is electronically tunable and allows for the rapid, accuracy and vibrationless selection of any wavelength in the range of 400～720 nm, and its fastest response time can be up to 50 ms. And the system follows the Camera Link specification and the PCI bus standard, which makes the embedded computer realize real-time control of the wavelength tuning of LCTF, the exposure time setting of the camera and the acquisition and storage of image data by program. The whole system characterized by small volume, low power consumption, high stability and high acquisition speed. The system could work on plane and airship platforms, and it′s suitable for researching in the fields of low altitude remote sensing and environmental monitoring.