Pollen Detection and Classification Method via Raman Spectroscopy

Xinyi Cao; Shangzhong Jin; Bin Hou; Zhihui Chen; Yun Wang

doi:10.3788/LOP57.133001

Journals >Laser & Optoelectronics Progress >Volume 57 >Issue 13 >Page 133001 > Article

Laser & Optoelectronics Progress
Vol. 57, Issue 13, 133001 (2020)

Pollen Detection and Classification Method via Raman Spectroscopy

Xinyi Cao¹, Shangzhong Jin^1、2、*, Bin Hou¹, Zhihui Chen¹, and Yun Wang¹

Author Affiliations

¹College of Optical and Electronic Technology, China Jiliang University, Hangzhou, Zhejiang 310018, China

²Key Laboratory of Zhejiang Province on Modern Measurement Technology and Instruments, Hangzhou, Zhejiang 310018, China

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DOI: 10.3788/LOP57.133001 Cite this Article Set citation alerts

Xinyi Cao, Shangzhong Jin, Bin Hou, Zhihui Chen, Yun Wang. Pollen Detection and Classification Method via Raman Spectroscopy[J]. Laser & Optoelectronics Progress, 2020, 57(13): 133001 Copy Citation Text

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Fig. 1. Raman spectroscopy of pollen of Ophiopogon japonicus excited by different wavelengths

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Raman spectroscopy pretreatment process of Salvia elata pollen. (a) Original Raman spectroscopy; (b) smoothing treatment; (c) baseline correction; (d) normalization

Fig. 2. Raman spectroscopy pretreatment process of Salvia elata pollen. (a) Original Raman spectroscopy; (b) smoothing treatment; (c) baseline correction; (d) normalization

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Fig. 3. Prediction results of interfamily pollen by SVM

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Fig. 4. Classification results of 6 Rosaceae pollen by SVM

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Fig. 5. Raman spectroscopy comparison of 5 typical Compositae pollen with Magnoliaceae (Michelia maudiae Dunn) and Pinaceae (Pinus parviflora)

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Fig. 6. Raman spectroscopy of Calendula

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Category label	Name	Families/Genus	Main Raman characteristic peak /cm^-1
1	Liriope spicata	Liliaceae/Liriope Lour	718,854,1001,1128,1185,1270,1358,1446,1602,1629
2	Lysimachia candida Lindl	Primulaceae Batsch Borkh/Lysimachia	495,598,1000,1031,1166,1251,1307,1362,1440,1602,1660
3	Saiviaofficinalis	Labiatae/Salvia Linn	621,643,716,858,1000,1034,1158,1307,1444,1602,1665
4	Rhododendron simsii Planch	Ericaceae Juss/Rhododendron L	622,1001,1035,1166,1027,1314,1447,1601,1663
5	Oleander	Apocynaceae Juss/Nerium L	623,1005,1036,1157,1206,1311,1448,1526,1601,1665
6	Pansy	Violaceae/Viola	384,498,624,1001,1034,1153,1201,1211,1311,1376,1148,1600,1663
7	Hibiscus	Malvaceae/Hibiscus	480,784,866,943,1005,1035,1084,1127,1265,1340,1454,1565,1611,1658
8	Sedum lineare	Crassulaceae/Sedum	624,647,724,1001,1034,1158,1311,1449,1528,1604,1666
9	Dahlia pinnate	Compositae/Dahlia	956,1000,1154,1193,1278,1351,1447,1517,1597
10	Wintersweet	Calycanthaceae/Chimonanthus	520,605,716,1001,1305,1362,1436,1566,1603,1656
11	Eustoma grandiflorum	Gentianaceae/Lisianthus russellianus	879,963,1005,1158,1193,1275,1444,1524,1600
12	Verbena hybrida	Verbenaceae/Verbena	622,850,1002,1035,1169,1321,1453,1603,1668
13	Aquilegia viridiflora	Ranunculaceae/Aquilegia	520,602,640,714,853,1002,1158,1307,1361,1448,1569,1660,1659
14	Michelia maudiae	Magnoliaceae/Amylus	715,838,1001,1033,1304,1360,1448,1603
15	Osmanthus fragrans	Rhinoceros/Osmanthus	720,1004,1157,1193,1308,1361,1445,1524,1605,1666
16	Ixora chinensis Lam	Rubiaceae/Genus longboat	556,708,842,1001,1030,1168,1202,1441,1603,1651
17	Flowering plum	Rosaceae/Peach	413,576,849,1005,1154,1447,1524,1573,1604,1653
18	Viburnum	Caprifoliaceae/Viburnum	337,415,547,1027,1170,1223,1310,1443,1513,1556,1604
19	Violet	Cruciferae/Violets	382,438,708,896,1001,1035,1097,1122,1152,1378,1450,1600,1666
20	Pomegranate	Punicaceae/Punica L	369,428,514,849,1002,1201,1260,1451,1583,1604,1661
21	Carnation	Caryophyllaceae/Dianthus L	413,517,565,639,832,1002,1180,1248,1361,1447,1568,1608,1657
22	Pinus parviflora	Pinaceae/Pinus	547,644,1170,1203,1265,1312,1439,1552,1607,1630
23	Hypericum monogynum	Gambaraceae/Hypericum	417,582,1005,1159,1247,1362,1443,1528,1579,1608,1652
24	Myosotis	Day lilies/Forget-me-nots	640,843,1001,1033,1065,1171,1225,1302,1361,1440,1604,1661
25	Snapdragon	Hyacinaceae/Snapdragon	645,718,860,1004,1158,1192,1445,1528,1604,1655
26	Yellow flag	Iris family/Genus iris	484,644,716,870,1005,1157,1191,1267,1446,1528,1605,1658
27	Cinnamomum camphora	Camphoraceae/Camphor	520,949,1001,1156,1188,1276,1443,1524,1601,1659
28	Oxalis triangularis purpurea	Sorrel family/Genus sorrel	622,1002,1032,1159,1201,1283,1308,1445,1528,1603,1664

Table 1. Main Raman characteristic peaks of 28 inter-family pollen samples

Principal component	PC1	PC2	PC3	PC4	PC5	PC6	PC7	PC8	PC9
Contribution rate /%	40.090	11.002	8.513	7.273	4.707	3.699	3.283	2.664	2.288

Table 2. Principal component contribution rate

Category label	Name	Families/Genus	Main Raman characteristic peak /cm^-1
1	Prunus lannesiana	Rosaceae/Sakura	417,643,976,1116,1171,1221,1605
2	Photinia serrulata	Rosaceae/Heather	411,577,850,1002,1079,1170,1224,1318,1366,1443,1605,1660
3	Rubus hirsutus Thunb	Rosaceae/Rubus	644,720,844,1000,1171,1231,1312,1447,1606,1660
4	White rose	Rosaceae/Rosaceae	574,873,1003,1156,1281,1444,1518,1655
5	Flowering almond	Rosaceae/Peach	411,577,1003,1075,1157,1278,1444,1525,1569,1607,1654
6	Rosa multiflora Thunb	Rosaceae/Rosaceae	642,872,1003,1156,1281,1443,1519,1603

Table 3. Main Raman characteristic peak positions of 6 intergeneric pollen samples

Xinyi Cao, Shangzhong Jin, Bin Hou, Zhihui Chen, Yun Wang. Pollen Detection and Classification Method via Raman Spectroscopy[J]. Laser & Optoelectronics Progress, 2020, 57(13): 133001

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