Spectrophotometric determination of the amount of zinc on the imprint left on hands by zinc coatings with 2-(5-Bromo-2-pyridylazo)-5-(diethylamino)-phenol (5-Br-PADAP) as a chromogenic reagent has been studied in this paper. The effect of reaction conditions including volume and pH of buffer solution as well as the volume of chromogenic reagent on the determination has also been studied. On the optimized condition, the standard curve of zinc has been established and the amount of zinc on the imprint left on hands by zinc coatings with different contact time and time elapse has been determined separately. As the results shown, the optimized reaction condition is 4 mL of boric acid and borax buffer solution(pH 8.0), 0.2 mL of 5-Br-PADAP with the concentration of 1 g·L-1 and 1 mL of Triton-X-100 with volume fraction of 10%. Under this circumstance, high linearity of zinc is followed between 0 and 14 μg and the regression equation of zinc is y=1.851 34x+0.002 29. The amount of zinc on the imprint left on hands by zinc coatings, ranging from 0.425 to 2.377 μg·cm-2, increases with contact time from 10 second to 5 min and varies insignificantly from 5 to 10 min. The amount of zinc left on hands declines sharply with time elapse from 0 h to 2 h, and then slowly from 2 to 7 h. The amount of zinc within 7 h is only 0.188 μg·cm-2, which is 90% lower than that of 0 h. Therefore, it is suggested that the trace metal detection should be conducted as soon as possible. Besides, the amount of zinc on the imprint left on hands by zinc coatings with different time elapse is not entirely comply with the intensity of imprint left by zinc coatings. This demonstrates that the amount of zinc on hands is not the only factor influencing the intensity of imprint on hands with different time elapse. Additionally, it also proves the hypothesis that zinc in the complex of zinc and protein can be captured and bonded by 5-Br-PADAP resorting to a stronger chelating capacity in the experiment for the first time. The application of the combined methods in a macro and micro view is useful for study in mechanism of influencing factors in trace metal detection, which lays foundations l for further researches.