In order to determine the repair role of laser in the damage induced by ultraviolet-B (UV-B) radiation, the effects of He-Ne laser (5 mW·mm^-2) irradiation on Ca²⁺-ATPase， Mg²⁺-ATPase and Na⁺/K⁺-ATPase activity in six days seedlings of wheat after enhanced UV-B (10.08 kJ·mm^-2·d^-1) radiated were studied. The results showed that the Ca²⁺-ATPase activity and Mg²⁺-ATPase activity under UV-B handling were lower than CK group(P<0.01), Na⁺/K⁺-ATPase in contrast (P＞0.05); the Ca²⁺-ATPase activity, Mg²⁺-ATPase and Na⁺/K⁺-ATPase activity under the He-Ne laser radiation were higher than the CK group(P<0.01); and by the use of He-Ne laser and UV-B handling the Ca²⁺-ATPase, Mg²⁺-ATPase activity were higher than the one under UV-B handling, but lower than CK group, Na⁺/K⁺-ATPase activity was higher than CK group. It suggested that He-Ne laser can improve ATPase activity. It indicated that the damage of wheat seedlings induced by UV-B radiation can be repaired partly by He-Ne laser.