In order to design and fabricate quarter wavelength high performance polarizing beam splitter (PBS),based on equivalent layers concept and stopband theoretics,two types quarter wavelength periodic multilayer PBS are proposed. The TFCalc software is used for the calculations and analysis. The polarizing beam splitters are fabricated by electron gun vapour deposition in spectrum range of 580-780 nm. The transmissivity characteristic of PBS is investigated by spectrometer,and the extinction ratio characteristic is measured by the constructed setup in the experiment. The experimental results indicated that the transmissivity reachs 90% or more,and the extinction ratio is better than 3×10-3. The experimental results consistent with theoretical performance of the layer design.