Hong–Ou–Mandel (HOM) interference is one of the most important experimental phenomena in quantum optics. It has drawn considerable attention with respect to quantum cryptography and quantum communication because of the advent of the measurement device independent (MDI) quantum key distribution (QKD) protocol. Here, we realize HOM interference, having a visibility of approximately 38.1%, using two independent heralded single-photon sources (HSPSs). The HOM interference between two independent HSPSs is a core technology for realizing the long-distance MDI QKD protocol, the quantum coin-tossing protocol, and other quantum cryptography protocols.