A non-gain microchannel plate (MCP) gated X-ray framing camera was reported. It is made up of four basic components, the non-gain MCP imager, the pinhole array, the picosecond gating pulse generator, and the CCD camera system. The temporal resolution was measured by using a fiber bunch. While the gating pulse with width of 145 ps and amplitude of -1.5 kV plus -300 V bias were applied on the non-gain MCP, the measured temporal resolution of this camera was about 59 ps. Furthermore, the relationship between the temporal resolution and the MCP bias voltage was obtained. The variation of the temporal resolution with the intensity of the output signal was also provided. The experimental results show that the temporal resolution is improved while the MCP bias voltage is decreased. However, the intensity of the output signal is reduced while the MCP bias voltage is decreased.