Based on the absorption spectra of hemoglobin and myoglobin in the near infrared band,the concentrations of these pigments in the biological tissues can be obtained using near infrared spectroscopy (NIRS) by detecting the intensity attenuation of the emitted light compared with the incident light. Based on the steady-state spatially resolved NIRS,the prototype for detecting the concentrations of tissue hemoglobin and myoglobin was independently developed by our group. The probe consisted of an LED light source which could emit three different wavelengths in the near infrared band,and two detectors which were placed on the same line with and at the distances of 30/40mm to the LED. The pigment concentrations of two pieces of pork,one from the erector spinae and the other from the rectus femoris,were detected using this prototype. The total concentrations of hemoglobin and myoglobin (ctotal) were (6.42±1.51)μmol·L-1 in the erector spinae,and (15.48±4.54)μmol·L-1 in the rectus femoris,respectively. The myoglobin was dominant in both of the results. These were consistent with the recent empirical reports. In summary,the NIRS method and prototype are authentic in detecting the pigment concentrations of pork tissue non-invasively,real-time,directly and conveniently.