Holographic data storage is a key research direction in the optical data storage field due to its high storage density, high capacity and high speed of parallel data accessing. Bacteriorhodopsin (BR), a biologically photochromic material, is a new kind of re-writable holographic recording media. The feasibility of angle multiplexing and polarization multiplexing holographic recording with BR film is proved experimentally. Based on the photochromic property of BR, using 90° angle multiplexing scheme, six holograms are recorded in the BR-D96N film and the reconstructed images are respectively read out clearly without crosstalk. Based on the photoanisotropic property of BR, polarization multiplexing holographic recording in the BR-D96N film is presented. Two images with orthogonal polarizations are recorded as two different holograms at the same position, respectively, which can be selectively retrieved with the original reference beam as readout beam and the appropriate polarizer.