As a novel optical communication material, quantum dot (QD)-doped glass became one of the focal researches in recent years. PbSe QD-doped sodium-aluminum-borosilicate glass is fabricated successfully by the melting method. The crystallization, size and distribution of the PbSe QD in the glass are investigated by X-ray diffractometer and transmission electron microscopy. The absorption and photoluminescence (PL) spectra of the PbSe QD doped-glass are also observed by UV-visible-NIR spectrophotometer and fluorescence spectrometer, respectively. The obtained results suggest that, when heat treatment temperature T is lower than 500 ℃, there is no observed PL emission in glass under irradiation of 1064 nm laser. When treatenent temperature T>550 ℃, the glass appears strong PL emissions with full width at half maximum (FWHM) in the range of 275~808 nm, and the PL peak wavelengths in 1676~2757 nm region strongly depended on the QD size. The size of PbSe QD is positively correlated with the heat treatment temperature, a Stokes shift of between the absorption and PL peaks is 20~110 nm.