In medicine and biology when the laser is used, the parameters, such as pulse duration, irradiation energy,pulse number and irradiation manner are very important. Light-absorbing nanoparticles that are heated by short laser pulses can transiently increase the membrane permeability. Different laser sources and irradiation manner were used to investigate the transfection efficiency accociated with the laser parameters. After irradiation, the memebrane permeability was evaluated by flow cytometry assaying of propidium iodide (PI) and fluorescein isothiocyanate (FITC) dextran. The mechanism of light-induced damage was studied by thermal modeling. The preliminary results suggest that this laser-induced permeability strategy provides a promising new means of delivering larger exogenous materials such as plasmid DNA into living cells by adjusting the laser parameter.