Fig. 1. Stationary absorption spectra of completely oxidized (black) and completely reduced (red) Cyt b₅

Fig. 2. UV-Vis absorption spectra of Cyt b₅ with/without irradiation at 280 nm
(a): Before illumination; (b): Upon illumination c_{cyt b5}=5×10^-6 mol·L^-1

Fig. 3. UV-Vis absorption spectra of chemical reduction for Cyt b₅ with sodium dithionite
(a): The absorption spectra of chemical reduction for Cty b₅ in soret band with sodium dithionite; (b): The obsorption spectra of chemical reduction for Cty b₅ in Q band with sodium dithionite c_{cyt b5}=3×10^-6 mol·L^-1, from a to e, cNa2S2O4=0, 3, 6, 9, 12×10^-6 mol·L^-1, respectively

Fig. 4. UV-Vis absorbance change of Cyt b₅ irradiated by xenon lamp at different fixed wavelengths
(a): 254 nm; (b): 280 nm; (c): 412 nm; (d): 423 nm

Fig. 5. Curve of irradiation reduction peak area of Cyt b₅ solution under different pH conditions at 280 nm
(a): The peak area changes at 412 nm; (b): The peak area changes at 556 nm

Fig. 6. UV-Vis absorbance change of Cyt b₅ added GSH under pH 7.4 a: Cyt b₅; b: Cyt b₅ irradiated at 25 min; c: Cyt b₅-GSH irradiated at 25 min

Fig. 7. UV-Vis absorbance change of Cyt b₅ added Met under pH 7.4 a: Cyt b₅; b: in dark 30 min; c: Cyt b₅ irradiated at 5 min; d: Cyt b₅-Met irradiated at 5 min

Fig. 8. UV-Vis absorbance change of Cyt b₅ added imidazole under pH 7.4
(a): The peak area changes at 412 nm; (b): Area changes at 556 nm

Fig. 9. Fluorescene spectra of Cyt b₅ at different irradiated time at 280 nm Frome a to f, the irradiation times were 0, 5, 10, 15, 20, 25 min, respectively

Fig. 10. CD spectra of Cyt b₅ after irradiation with xenon lamp at 280 nm From a to c, the irradiation times were 0, 10, 25 min, respectively