A new method for measuring fluorescence quantum efficiencies of active ions in Nd3+: glass is described. According to the principle of photoaooustic signal, the fluorescence quantum efficiencies has been calculated with the measured values of absorption amplitudes at two absorption peaks and the amplitudes of photoacoustic signals at the same wavelengthes for the Nd3+ ions in Nd3+: glass. The obtained results are in good agreement with those calculated and measured by other methods.