A simple protocol to simultaneously determinate chiral enantiomers without separation is of great significance. Herein, we reported a method for chiral recognition was proposed. Cu2+ functionalized gold nanoparticles (Au NPs) was used as a sensor for carnitine enantiomers based on the resonance Rayleigh Scattering (RRS) spectral technique. The RRS intensity of Au NPs is very weak, while RRS highly increased in the presence of Cu2+. More interestingly, carnitine enantiomers could quench the RRS intensities of Cu2+-Au NPs system, and they had different quenching degree. In the optimum experimental conditions, good linear relation, high correlation coefficient and low detection limit could be obtained. In addition, this proposed method could obtain enantiomer ratios and enantiomer fraction of carnitine enantiomers. And the applicability of the chiral recognition of carnitine enantiomers mixtures in capsule samples had been demonstrated. Particularly, this method does not require complicated chiral modification and excels through its low-cost, sensitivity, enantioselectivity, simplicity and good availability of materials.