Optical polarimetry for probing aqueous humor glucose concentrations has been shown as a method for ascertaining blood glucose concentrations noninvasively. One major limiting factor for polarimetric approach for glucose sensing is time-variant corneal birefringence due to motion artifact. A real-time closed-loop single wavelength polarimetric glucose sensor is designed utilizing modulation of laser simultaneously with Faraday modulation. The method overcomes the problem of previous polarimetric systems whose response speed is solely limited by the Faraday modulation frequency, when the lasers are powered by a constant direct-current voltage and the polarization is modulated using a single Faraday rotator. It makes the feedback system reach stable status within 10 ms, which should be fast enough to minimize the effect of polarization rotation due to birefringence. This approach can reduce laser noise from 1/f in addition to electromagnetic interference, improving the signal-to-noise ratio of the system at the presence of motion artifact. The system is constructed and evaluated in vitro for glucosedoped water solutions, whose concentrations are 0~600 mg/dL with increment of 100 mg/dL, the standard error of predicted results of glucose concentration is less than 10 mg/dL, demonstrating the repeatability and accuracy of the system.