A 45° tilted fiber grating working at 1064 nm is fabricated successfully in photosensitive fiber by ultraviolet (UV) inscription technique. A phase mask with a period of 1070 nm is rotated by 32.7° to the fiber axis to produce tilted fringes of 45° in the fiber core. The length of the 45° tilted fiber grating is about 24 mm. The polarization dependent loss (PDL) characteristics of the tilted grating are experimentally researched. The PDL value of the 45° TFG is about 11.8 dB at 1064 nm and it keeps higher than 9 dB over a wavelength range of 50 nm from 1150 nm to 1110 nm. According to the theoretical analysis, the main causes of the relatively smaller PDL value of the tilted grating are that the period of the phase mask is not the optimum value and the length is not enough.