In the analysis of Raman spectroscopy, the sample and contaminants often produce strong fluorescence signal which seriously affects the detection of Raman spectrum signal produced by the sample, and limits the application of the technology. In recent years, a number of methods have been developed to solve the problem of fluorescence interference. In this paper, the fundamental principle, implementation and performance characteristics of various methods were reviewed and summarized, and the application of several common fluorescence suppression methods(fluorescence quenching method, light bleaching method, ultraviolet light excitation method, infrared light excitation method, shifted-excitation Raman difference spectroscopy and wavelet transform)were also concretely analyzed in different fields including excitation wavelength, fluorescence source, variation of fluorescence signal, the effect of fluorescence suppression, the influence of the Raman signal and so on. It not only shows the advantages of each method, but also illustrates its disadvantages.