-ray fluorescence spectrum micro-area analysis method has the characteristics of rapid, simple and non-destructive testing, and can detect the distribution of elements on the surface of health food. Inductively coupled plasma mass spectrometry has the advantages of low detection limit, wide linear range and multi-element simultaneous determination. Methods: A health food was analyzed by X-ray fluorescence spectrum micro-region analysis technique, and the elemental content was semi-quantitatively analyzed to determine the elements of calcium (Ca), iron (Fe), ruthenium (Ru) and molybdenum (Mo) in health products. The main elements of Ca and Fe were quantitatively analyzed by microwave digestion-inductively coupled plasma mass spectrometry (ICP-MS). The average value of Ca element was 6.23%, the relative standard deviation was 1.78%, the average value of the Fe element was 3.82%, and the relative standard deviation was 2.14%. The results were consistent with the conclusion that the content of Ca in the sample with X-ray fluorescence spectrum micro-analysis was between 6.0% and 10.0%, and the content of Fe in the sample was between 2.0% and 4.0%. The distribution of the elements on the surface of health food was not uniform by X-ray fluorescence spectrum analysis. The X-ray fluorescence spectrum micro-area analysis method cannot only measure the elemental semi-quantitative content in health food by using the trace sample, but also measure the elemental distribution in health food. Combined with inductively coupled plasma mass spectrometry, the content of the elements of interest in the sample can be further detected, and the quantitative analysis results can be obtained.