One differential absorption lidar (DIAL) for measuring NO2 concentration profile is developed by using atmospheric backscattered signals. Two Nd∶YAG lasers are used to pump two dye lasers to produce two laser beams with the wavelengths of λon and λoff, respectively. Retrieval of acquired data provides the vertical and the horizontal distributions of NO2 concentration. The experimental results show that the volume fraction of NO2 at Huainan Institute of Atmospheric Sciences, fluctuates in the range from 0 to 2.5×10-8 in the atmospheric vertical height range of 0.4-3.0 km and 0 to 3.0×10-8 in the horizontal distance range of 0.4-3.0 km, respectively.