Photoacoustic spectroscopy technology (PAST) is a new technology for researching material absorption spectra; it has become an important branch in the molecular spectroscopy. As a powerful tool for analysis in the field of modern biomedical, Photoacoustic spectroscopy technology overcomes the influence of the biologic tissue′s scatter characteristics, providing an effective and noninvasive way for biomedical materials research, being highly sensitive and without sample pretreatment. The basic principle of photoacoustic spectroscopy technology and the experimental devices are described and the applications of photoacoustic spectroscopy technology in the modern biomedicine are introduced.