On the basis of study about fluorescence spectra of alcohol-water mixtures and their characteristic, by using SP-2558 multifunctional spectral measuring system, the fluorescence spectra of more than 50 kinds of liquors excited by ultraviolet (UV) light have been measured. The results show that a stronger fluorescence can be generated when liquor is excited by UV light, and its characteristic is clear. Three-dimensional fluorescence spectra of various liquor were measured. Based on this, a fluorescence spectra fingerprint atlas of liquors was constructed. Through analysis, a spectra database of liquors including the number of peaks, the peak wavelength, best exciting wavelength, etc. was established. By using SGL Server 2005 and VB 6.0, an intelligent identification system of liquors was created. The fluorescence spectra of unknown liquor can be automatically compared with those of known liquor in the database, and this system can intelligently identify liquor and get degree of similarity. Fluorimetry has high sensitivity and accuracy, its sample is a little and need not pre-treatment, this work bring the advantages of fluorescence spectroscopy into play, the research results provide an effective new method for identification of liquors, it can be applied to production management and market supervision of liquor, and provide helps for food safety.