A new type of hyperbranched polyamide grafted chitosan (CS-PAMAM) adsorbent was synthesized using glutaraldehyde (GLA) as the crosslinker. The adsorbent wascharacterized with Field Emission Scanning Electron Microscope and Fourier Transform Infrared. Combined with flame atomic absorption spectroscopy (FAAS) and UV-Vis spectrophotometry, the adsorption of Malachite green (MG), Sunset yellow (SY) and Cu(Ⅱ) by adsorbent were investigated. Experimental conditions affecting the adsorption percentage were discussed and the experimental operation parameters were optimized (pH value were 7.0, 2.0 and 6.0, respectively; adsorption time were 60, 60 and 30 min, respectively). Under the optimal condition, the maximum adsorption capacity of MG, SY and Cu(Ⅱ) on CS-PAMAM was found to be 515.30, 201.79 and 80.00 mg·g-1, respectively. The advantages are obvious. The adsorption process for MG, SY and Cu(Ⅱ) obeyed Langmuir isother mequation.