• Laser & Optoelectronics Progress
  • Vol. 60, Issue 13, 1316015 (2023)
Yankun Qi, Zhihao Zhang, Lü Shichao, and Shifeng Zhou*
Author Affiliations
  • State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou 510640, Guangdong, China
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    DOI: 10.3788/LOP230987 Cite this Article Set citation alerts
    Yankun Qi, Zhihao Zhang, Lü Shichao, Shifeng Zhou. Multifunctional Optical Fibers for Optogenetics[J]. Laser & Optoelectronics Progress, 2023, 60(13): 1316015 Copy Citation Text show less
    Schematic diagram of neural probe using laser as light source, optical fiber as optical signal transmission channel, and metal electrode wire as electrical signal channel[18]
    Fig. 1. Schematic diagram of neural probe using laser as light source, optical fiber as optical signal transmission channel, and metal electrode wire as electrical signal channel[18]
    Schematic illustration of the fabrication of integrated probe. (a) Design of prefabricated bars for composite materials[34]; (b) schematic diagram of hot stretching process principle[35]; (c) preparation of a typical hydrogel probes[34]; (d) schematic diagram of femtosecond laser micromachining process[36]
    Fig. 2. Schematic illustration of the fabrication of integrated probe. (a) Design of prefabricated bars for composite materials[34]; (b) schematic diagram of hot stretching process principle[35]; (c) preparation of a typical hydrogel probes[34]; (d) schematic diagram of femtosecond laser micromachining process[36]
    Schematic diagram of the multifunctional probe prefabrication and the application of a living mouse. (a) Schematic diagram of fabrication of optical fiber probe precast; (b) electrophysiological signals at 2 days, 1 week, 1 month, and 2 months after the probe was implanted in the mPFC of mice and subjected to synchronous photogenetic stimulation[27]
    Fig. 3. Schematic diagram of the multifunctional probe prefabrication and the application of a living mouse. (a) Schematic diagram of fabrication of optical fiber probe precast; (b) electrophysiological signals at 2 days, 1 week, 1 month, and 2 months after the probe was implanted in the mPFC of mice and subjected to synchronous photogenetic stimulation[27]
    Schematic diagram of the preparation of an all-polymer neural probe and its good biocompatibility.(a) Fabrication and thermal stretching of prefabricated rods including recording electrodes, optical waveguides, and microfluidic channels; (b) confocal microscope images of glial scar formation and blood-brain barrier breach around the implanted probe and stainless steel microwires at 1 month respectively (scale bar is100 μm)[37]
    Fig. 4. Schematic diagram of the preparation of an all-polymer neural probe and its good biocompatibility.(a) Fabrication and thermal stretching of prefabricated rods including recording electrodes, optical waveguides, and microfluidic channels; (b) confocal microscope images of glial scar formation and blood-brain barrier breach around the implanted probe and stainless steel microwires at 1 month respectively (scale bar is100 μm)[37]
    The adaptive bending stiffness of hydrogel hybrid probes and its high matching with mechanical properties of nerve tissue. (a) Description of the concept of adaptive bending stiffness; (b) Mises stress distribution of stainless steel, silica, PC fibers, and hydrogel hybrid probes in brain tissue during lateral micromotion[34]
    Fig. 5. The adaptive bending stiffness of hydrogel hybrid probes and its high matching with mechanical properties of nerve tissue. (a) Description of the concept of adaptive bending stiffness; (b) Mises stress distribution of stainless steel, silica, PC fibers, and hydrogel hybrid probes in brain tissue during lateral micromotion[34]
    Schematic diagram of the design of LOEF and the demonstration of its light leakage. (a) Three functions of LOEF; (b) schematic diagram of the principle of light leakage by laser ablation of the micro-window; (c) different light leak intensity from a pattern of a single, 1×10, and 2×10 micro-windows (from right to left); (d) image of fluorescent nanobeads in gelatin surrounding the LOEF showing the spatial distribution of light leak[30]
    Fig. 6. Schematic diagram of the design of LOEF and the demonstration of its light leakage. (a) Three functions of LOEF; (b) schematic diagram of the principle of light leakage by laser ablation of the micro-window; (c) different light leak intensity from a pattern of a single, 1×10, and 2×10 micro-windows (from right to left); (d) image of fluorescent nanobeads in gelatin surrounding the LOEF showing the spatial distribution of light leak[30]
    Schematic diagram of the multifunctional neural probe to simplify optogenetic experiments. (a) Schematic comparison of two-step operation performed by photogenetic experiment and one-step operation performed by multifunctional fiber probe; (b) schematic illustration of simultaneous viral delivery, optical stimulation and electrical recording in mPFC of mice with fiber probe; (c) integration of fiber probe photoelectric function with microfluidic channels[34,37]
    Fig. 7. Schematic diagram of the multifunctional neural probe to simplify optogenetic experiments. (a) Schematic comparison of two-step operation performed by photogenetic experiment and one-step operation performed by multifunctional fiber probe; (b) schematic illustration of simultaneous viral delivery, optical stimulation and electrical recording in mPFC of mice with fiber probe; (c) integration of fiber probe photoelectric function with microfluidic channels[34,37]
    Yankun Qi, Zhihao Zhang, Lü Shichao, Shifeng Zhou. Multifunctional Optical Fibers for Optogenetics[J]. Laser & Optoelectronics Progress, 2023, 60(13): 1316015
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