It is a relatively new task to apply the laser induced breakdown spectroscopy (LIBS) to fruit samples. To apply LIBS technique in the field related to analysis of trace heavy metal element in fruits samples, we have done primary experiments using Gannan navel orange samples. The authors put the samples into different concentration gradient K2Cr2O7 solution and left it for 30 hours, and then we did the LIBS experiment, discriminated characteristic spectra of chromium element and recorded the peak intensity information. Weighing three grams of sample and determined chromium concentration in the samples by atomic absorption spectrophotometer using wet digestion. The calibration curve of the line intensities versus the concentrations of the Cr element was acquired by the Origin software. The authors found that the linear correlation coefficient is 0.981 66. The calibration curve can be used for the quantitative analysis of chromium element with an unknown concentration in Gannan navel orange. The LIBS detection limit of Cr in the solution was 11.64 mg·g-1 from the measured calibration curve. Experiment results showed that LIBS technique is a valid means for measuring and quantitatively analyzing the content of heavy metal elements in fruit samples.