A pin-like beam is a kind of structured light with a special intensity distribution that can be against diffraction, which can be seen as a kind of quasi-nondiffracting beam (Q-NDB). Due to its wide applications, recently, numerous researchers have used optical lenses or on-chip integrated optical diffractive elements to generate this kind of beam. We theoretically verify and experimentally demonstrate an all-fiber solution to generate a subwavelength inverted pin beam by integrating a simple plasma structure on the fiber end surface. The output beams generated by two kinds of plasma structures, i.e., nanoring slot and nanopetal structure, are investigated and measured experimentally. The results show that both the structures are capable of generating subwavelength beams, and the beam generated using the nanopetal structure has the sidelobe suppression ability along the x-axis direction. Our all-fiber device can be flexibly inserted into liquid environments such as cell cultures, blood, and biological tissue fluids to illuminate or stimulate biological cells and molecules in them. It provides a promising fiber-integrated solution for exploring light–matter interaction with subwavelength resolution in the field of biological research.