The absolute spectral power distribution and net emission photon distribution of Er3+/Yb3+ doped waveguide-type aluminum germanate glasses are investigated, and the absolute quantification of upconversion fluorescence parameters of rare earth ion are realized. The performance of Er3+ ion upconversion fluorescence is characterized by integrating sphere and charge-coupled device detector system. The absolute spectral power distribution of upconversion fluorescence is analyzed. Further, the fluorescence parameters such as the number of the net emission photon and the quantum yields are derived. The results show that the effective diffusion rate of K+-Na+ ion-exchange in the glass sample of Er3+/Yb3+ doped aluminum germanate glasses in KNO3 fused salt at 380 ℃ is 0.077 μm2/min. The green light and red light centered at 548 nm and 661 nm are emitted after Er3+ illuminated by 975 nm pump light, and the red light is dominant emission. The output power and the net emission photon of dominant red emission are 47.86 μW and 16.03×1013 s-1 respectively, when the laser power density is 1227 W/cm2. Compared with the relative measurement method whose fluorescence quantum yield magnitude in germanate glass stays within the range of 10-6-10-5, absolute method for photon quantification can provide more accurate data, which significantly improves the accuracy and repeatability of test parameters for rare earth upconversion fluorescence in waveguide-type aluminum germanate glasses.