Feasibility of using two-point calibration method to acquire aerosol extinction-to-backscattering ratio is explored. Two-point calibration method is one of the important methods for acquiring aerosol extinction-to-backscattering ratio. The relationship between the distance of the two calibration points and the accuracy of aerosol extinction-to-backscattering ratio acquired by using two-point calibration method is discussed. The aerosol extinction-to-backscattering ratio obtained by using two-point calibration method is reliable when the distance between the two points is greater than 1 km. The influence of the distance between the two calibration points on the inversion results of aerosol extinction-to-backscattering ratio from one calibration point to another one is analyzed. The influence to aerosol extinction-to-backscattering ratio inversion results of two different iterative methods is discussed. The feasibility of beforehand error estimation of inversion results when two-point calibration method is used for the inversion of aerosol extinction-to-backscattering ratio is investigated. The inversion results show that the error of aerosol extinction-to-backscattering ratio between the two calibration points does not exceed 6% generally when the distance between the two calibration points is greater than 1 km and the extinction coefficient error of the calibration point is 5%.