The theory of Near Field Optical Microscopy (NFOM) is reviewed. A real system is presented with which the fish scale sample is imaged and analyzed. The results show that NFOM can be applied to study transparent and semi- transparent materials,especially, for living materials. Bioelectret properties caused by orderly orientational arrangement of collagen molecules is proved. By NFOM the bioholographic phenomenon is observed for the first time in μm-sized fish scale plates.