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Imaging biological tissues using sonoluminescence (SL) is a new research field developing in the latest two years. Sonoluminescence image of living body (nude mouse) was obtained by the high quantum efficiency imaging system. Fluoresceinyl Cypridina Luminescent Analog (FCLA), a chemiluminescent analytic agent, is used in vivo for the first time to enhance biological SL. An image of FCLA enhanced SL in vivo was observed too. This technique has potential application in clinics for imaging diagnosis.