Combining light field microscope and naked-eye three-dimensiional display technique, a new method to realize real-time naked-eye observation of micro-specimen in a light field microscope is proposed. The sub-image array obtained by a light field microscope is projected on the focal plane of microlens array directly. Observers can see two perspective views of the specimen at the same time in a certain region and feel stereo effect because of binocular. The proposed method has advantageous properties including simple configuration, no requirement of coherent light source and allowing many people observing at the same time without wearing special glasses, and thus it has an important prospect of application.