Fourier transform infrared spectroscopy (FTIR), second derivative infrared spectroscopy, and two-dimensional correlation infrared spectroscopy (2D-IR) were used to study the steaming procedure of aconite tuber. The results showed that samples with steaming procedures were similar to each other in one-dimensional IR spectra but different in 2D-IR spectra. The FTIR spectra combined with second derivative infrared spectroscopy of different samples during boiling procedure present characteristic peaks at 1　63487 cm-1 (CO), 1　60318, 1　57158 cm-1, 1　48509, 1　41308 cm-1 (vibration of phenyl framework) and 1　15373, 1　08184, 1　02135 cm-1 (C—O) respectively. There were two conspicuous time sequences in two dimensional infrared correlation spectroscopy of autopeaks intensity. Since, the intensity of autopeaks was relatively high during 2～3 and 8～9　h. The function of FTIR and 2D-IR for analysis of the alkaloid from aconite root attributed high speed and high efficiency. The results were reliable and valuable.