Purposed to achieve the detection of changes in tissue structure and composition simultaneously by virtual internal hyper-spectrum of body surface (VIHBS), we designed the VIHBS system containing modulated light, translation stage device for data acquisition and spectrometer. In the present study, pork meat as the experimental subject was equally divided into three pieces. One piece was used for a control study, and the others were separately embedded with red filter and injected into highly scattering intralipid to imitate the changes in tissue structure and composition. And then, data acquisition of 17 points started at source fiber was taken at intervals of 0.5 mm. The results showed that modulated light can effectively inhibit the influences of ambient light and dark current created by optoelectronic devices. In addition, VIHBS technology achieved the locating of red filter by visible-light and the rapid screening of changes in tissue composition caused by intralipid with near infrared light. The study suggests that it is much feasible to detect the changes in tissue structure and composition by VIHBS. And it is concluded that further research will be likely to provide a new method to realize the early diagnosis and screening of human skin.