Normalization of Quantitative Analysis for Bacteria Multi-Wavelength Transmission Spectroscopy in Water

Yuxia Hu; Nanjing Zhao; Tingting Gan; Jingbo Duan; Deshuo Meng; Jianguo Liu; Wenqing Liu

doi:10.3788/AOS201838.0430001

Journals >Acta Optica Sinica >Volume 38 >Issue 4 >Page 0430001 > Article

Acta Optica Sinica
Vol. 38, Issue 4, 0430001 (2018)

Normalization of Quantitative Analysis for Bacteria Multi-Wavelength Transmission Spectroscopy in Water

Yuxia Hu^1、2、3, Nanjing Zhao^1、3、*, Tingting Gan^1、3, Jingbo Duan^1、3, Deshuo Meng^1、3, Jianguo Liu^1、3, and Wenqing Liu^1、3

Author Affiliations

¹ Key Laboratory of Environment Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei, Auhui 230031, China

² University of Science and Technology of China, Hefei, Auhui 230026, China

³ Anhui Key Laboratory of Environment Optical Detection Technology, Hefei, Anhui 230031, China

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DOI: 10.3788/AOS201838.0430001 Cite this Article Set citation alerts

Yuxia Hu, Nanjing Zhao, Tingting Gan, Jingbo Duan, Deshuo Meng, Jianguo Liu, Wenqing Liu. Normalization of Quantitative Analysis for Bacteria Multi-Wavelength Transmission Spectroscopy in Water[J]. Acta Optica Sinica, 2018, 38(4): 0430001 Copy Citation Text

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Fig. 1. Transmission spectra of Escherichia coli suspension with three concentrations

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(a) Measured and calculated optical density of spectrum 1 after particle concentration normalization; (b) concentration fitting curves of three different transmission spectra

Fig. 2. (a) Measured and calculated optical density of spectrum 1 after particle concentration normalization; (b) concentration fitting curves of three different transmission spectra

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Fig. 3. (a) Measured and calculated optical density of spectrum 1 after maximum normalization; (b) concentration fitting curves of three different transmission spectra

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Fig. 4. (a) Measured and calculated optical density of spectrum 1 after integral normalization; (b) concentration fitting curves of three different transmission spectra

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Fig. 5. (a) Measured and calculated optical density of spectrum 1 after average normalization; (b) concentration fitting curves of three different transmission spectra

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Spectrum	Particle concentration normalization		Maximum normalization		Integral normalization		Average normalization
Spectrum	$\begin{matrix} {D_{1}}_{} \end{matrix}$ /μm	$\begin{matrix} {D_{2}}_{} \end{matrix}$ /μm	$\begin{matrix} {D_{1}}_{} \end{matrix}$ /μm	$\begin{matrix} {D_{2}}_{} \end{matrix}$ /μm	$\begin{matrix} {D_{1}}_{} \end{matrix}$ /μm	$\begin{matrix} {D_{2}}_{} \end{matrix}$ /μm	$\begin{matrix} {D_{1}}_{} \end{matrix}$ /μm	$\begin{matrix} {D_{2}}_{} \end{matrix}$ /μm
1	1.4900	0.0500	1.7571	0.0501	1.2792	0.0501	1.2715	0.0501
2	1.3900	0.0500	1.4351	0.0501	1.4635	0.0501	1.4602	0.0501
3	1.4900	0.0500	1.4799	0.0501	1.4086	0.0525	1.4086	0.0500

Table 1. Structural parameters of Escherichia coli obtained with the inversion of different normalization transmission spectra

No.	Concentration from plate counting /(10⁷ mL^-1)	Particle concentration normalization		Maximum normalization		Integral normalization		Average normalization
No.	Concentration from plate counting /(10⁷ mL^-1)	Concentration/(10⁷ mL^-1)	Error /%	Concentration/(10⁷ mL^-1)	Error /%	Concentration/(10⁷ mL^-1)	Error /%	Concentration/(10⁷ mL^-1)	Error /%
1	3.4200	2.3668	30.80	1.0008	70.74	3.3512	2.01	3.4300	0.29
2	2.5200	3.0712	21.87	2.7175	7.84	2.5248	0.19	2.5432	0.92
3	3.5000	2.7766	20.67	2.9230	16.49	3.5424	1.21	3.5310	0.89
Average relative error /%		24.45		31.69		1.14		0.70

Table 2. Concentrations obtained with four normalization methods and serial dilution plate counting method

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