Xiao-xia WANG, Hao WU, Zhi-hua NIE, Li-tong MA, Jin-long CUI, Hua-zheng SAI, Jian-guo CHENG. Study on the Interaction Between Fulvic Acid and Bovine Serum Albumin by Multispectral and Molecular Docking[J]. Spectroscopy and Spectral Analysis, 2021, 41(9): 2904
- Spectroscopy and Spectral Analysis
- Vol. 41, Issue 9, 2904 (2021)
Fig. 1. Fluorescence emission spectra of BSA-FA interaction system under different temperature
(a): 298 K; (b): 303 K; (c): 308 K;c (BSA)=5×10-7 mol·L-1;c (FA)(1—9): (0, 0.6, 1.2, 1.8, 2.4, 3.0, 3.6, 4.2, 4.8)×10-4 mol·L-1; pH 7.40
(a): 298 K; (b): 303 K; (c): 308 K;
Fig. 2. Overlapping of the fluorescence emission spectra (a ) of BSA and with the absorption spectra (b ) of FA
c (BSA)=c (FA)=5.0×10-7 mol·L-1
Fig. 3. The molecular docking result of FA with BSA
(a): Molecular docking simulation of FA and BSA; (b): Streamer shape molecular docking model diagram of FA and BSA;(c): 2D and 3D schematic diagram of amion residues of FA and BSA
(a): Molecular docking simulation of FA and BSA; (b): Streamer shape molecular docking model diagram of FA and BSA;(c): 2D and 3D schematic diagram of amion residues of FA and BSA
Fig. 4. UV-Visible absorption spectroscopy of FA-BSA interaction system
c (BSA)=5×10-7 mol·L-1; c (FA)(1—9): (0, 0.6, 1.2, 1.8, 2.4, 3.0, 3.6, 4.2, 4.8)×10-4 mol·L-1; pH 7.40; T =298 K
Fig. 5. Synchronous fluorescence spectrogram of FA-BSA interaction system
c (BSA)=5×10-7 mol·L-1; c (FA)(1—9): (0, 0.6, 1.2, 1.8, 2.4, 3.0, 3.6, 4.2, 4.8)×10-4 mol·L-1; pH 7.40; T =298 K; (a): Δλ =15 nm, (b): Δλ =60 nm
Fig. 6. Three-dimensional fluorescence spectrum and contour map of BSA
c (BSA)=2.5×10-7 mol·L-1; pH 7.40; T =298 K
Fig. 7. Three-dimensional fluorescence spectrum and contour map of FA-BSA
c (BSA)=2.5×10-7 mol·L-1;c (FA)=3.0×10-5 mol·L-1; pH 7.40; T =298 K
Fig. 8. The circular dichroism spectra of BSA and FA-BSA interaction system
(a):c (BSA)=1.0×10-5 mol·L-1; (b): c (BSA)=1.0×10-5 mol·L-1, c (FA)=4.0×10-5 mol·L-1; T =298 K; pH 7.40
(a):
|
Table 1. Stern-Volmer linear equations and correlation coefficients
Set citation alerts for the article
Please enter your email address
© Copyright 2018-2021 | Chinese Laser Press. All Rights Reserved 沪ICP备15018463号-20