Qiu Zhang, Dong Liang, Lihua Bai, Jun Liu. Long-Time Stabilization Imaging by Light Sheet Fluorescent Microscope[J]. Chinese Journal of Lasers, 2019, 46(4): 0407001
- Chinese Journal of Lasers
- Vol. 46, Issue 4, 0407001 (2019)
Fig. 1. Schematic of long time stabilization system with 4f optical setup and CCD camera
Fig. 2. Schematic of LSFM experimental setup
Fig. 3. Control signal sequences for sCMOS camera as well as GM and PZE/NS
Fig. 4. Flow chart for imaging under feedback control
Fig. 5. Full width at half maximum of light sheet in CCD camera. (a) N=1; (b) N=5/2; (c) N=5/3
Fig. 6. Movement of light sheet in CCD camera under step size of 25 nm
Fig. 7. Mechanical drifts in z axis of sample under different conditions
Fig. 8. Effect of mechanical drift on imaging. (a) Effect of mechanical drift in direction perpendicular to imaging plane; (b) effect of mechanical drift in plane
Fig. 9. Imaging effects of fluorescent microspheres under different conditions. (a)-(d) Without feedback; (e)-(h) with feedback
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